Test samples of the finished product for performance using stable, typical control cultures. Warm gently until solution is complete.Ĥ. Trypticase Soy Broth – 30 g Mix thoroughly.Ģ. Suspend the powder in 1 L of purified water: Directions for Preparation from Dehydrated Productġ. *Adjusted and/or supplemented as required to meet performance criteria. 2.5 gīBL™ Trypticase™ Soy Broth (Soybean-Casein Digest Broth) ORGANISMīacto™ Tryptic Soy Broth (Soybean-Casein Digest Medium) albicans and incubate at 20-25☌ for up to 3 days (up to 5 days for A. albicans). Prepare duplicate cultures of A. Inoculate tubes and incubate at 30-35☌ for up to 3 days (up to 5 days for A. Inoculate and incubate at 30-35☌ for 18-72 hours (up to 5 days for A. Prepared Appearance: Light, tan to yellow, clear to slightly hazy. Solution: 3.0% solution, soluble in purified water upon warming.
BACTO TRYPTIC SOY BROTH FREE
Solution: 3.0% solution, soluble in purified water uponĭehydrated Appearance: Fine, homogeneous, free of extraneous material. In the development and testing of media for industrial and clinical applications, per the referenced publications.ĭehydrated Appearance: Light beige, free-flowing, homogeneous. NOTE: Differences in the Identity Specifications and Cultural Response testing for media offered as both Difco™/Bacto™ and BBL™ brands may reflect differences All varieties of bottled TSB conform with requirements for Ready- To-Use Media as described in the USP. In addition, Tryptic Soy Broth is provided as a Sterile Pack Bottle i.e., the bottle has been terminally sterilized inside of autoclavable double-bags. Tryptic Soy Broth and Trypticase Soy Broth are provided as prepared media in a variety of bottle styles. Dibasic potassium phosphate acts as a buffer to control pH. Sodium chloride maintains the osmotic equilibrium. 17,18 Principles of the ProcedureĮnzymatic digests of casein and soybean provide amino acids and other complex nitrogenous substances. 10-16īecause of its capacity for growth promotion, TSB is also recommended for use as the inoculum broth for disc diffusion and agar dilution antimicrobial susceptibility testing as standardized by the Clinical and Laboratory Standards Institute (CLSI). 8 TSB is recommended for testing bacterial contaminants in cosmetics 9,10 and complies with established standards in the food industry. TSB was chosen by the USDA Animal and Plant Health Inspection Service for detecting viable bacteria in live vaccines. 4-7 This formulation is included in the USP as a medium for use in performing microbial enumeration tests and tests for specified microorganisms when testing nonsterile pharmaceutical products. Tryptic (Trypticase) Soy Broth (TSB) is a nutritious medium that will support the growth of a wide variety of microorganisms, including common aerobic, facultative and anaerobic bacteria and fungi.
Tryptic (Trypticase) Soy Broth meets United States Pharmacopeia (USP), European Pharmacopoeia (EP) and Japanese Pharmacopoeia (JP)1-3 performance specifications, where applicable.
Because of its capacity for growth promotion, TSB is also recommended for use as the inoculum broth for disc diffusion and agar dilution antimicrobial susceptibility testing as standardized by the Clinical and Laboratory Standards Institute (CLSI).Tryptic (Trypticase) Soy Broth (Soybean-Casein Digest Medium) is a general purpose medium used in qualitative procedures for the cultivation of fastidious and nonfastidious microorganisms from a variety of clinical and nonclinical specimens.TSB is recommended for testing bacterial contaminants in cosmetics and complies with established standards in the food industry.TSB was chosen by the USDA Animal and Plant Health Inspection Service for detecting viable bacteria in live vaccines.This formulation is included in the USP as a medium for use in performing microbial enumeration tests and tests for specified microorganisms when testing nonsterile pharmaceutical products.TSB is a nutritious medium that will support the growth of a wide variety of microorganisms, including common aerobic, facultative and anaerobic bacteria and fungi. Trypticase Soy Broth (Soybean-Casein Digest Medium) is a general purpose medium used in qualitative procedures for the cultivation of fastidious and non-fastidious microorganisms from a variety of clinical and nonclinical specimens.
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